李宗鲜,许明珠,吉木伍各木,龚思月,呼海燕

• 1:成都医学院
• 2:四川护理职业学院

摘要(Abstract)：

目的观察生长分化因子11(GDF11)对RAW264.7细胞增殖、分化及破骨细胞相关转录因子RANK、肿瘤坏死因子相关受体因子(tumor necrosis factor receptor-associated factors,TRAF6)和p38mRNA的表达。方法培养RAW264.7细胞,通过CCK-8法、TRAP染色、q-PCR技术及骨吸收实验测定细胞增殖及RANK、TRAF6和p38mRNA表达。结果体积浓度为0.1、1、2ng/mL的GDF11促进细胞增殖;GDF11促进RANK、TRAF6和p38 mRNA表达;GDF11、GDF11+RANKL和RANKL使TRAP活性明显增加;GDF11、GDF11+RANKL和RANKL均使破骨样细胞的骨吸收能力增强。结论 GDF11可能通过增加RANK、TRAF6和p38mRNA表达,促进破骨样细胞增殖和提高TRAP活性,并促进破骨细胞的骨吸收作用。

关键词(KeyWords)： RAW264.7细胞;GDF11;RANKL;信号通路;骨吸收

Abstract:

Keywords:

基金项目(Foundation): 四川省教育厅自然科学基金项目(No:17ZA0115);; 国家大学生创新创业训练计划项目(No:201813705035,No:201813705034)

作者(Author): 李宗鲜,许明珠,吉木伍各木,龚思月,呼海燕

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